The Shandon Cytospin was the first device to use low-speed centrifugal force to separate and deposit a monolayer of cells on slides while maintaining cellular integrity.
 

Sample Requirements:

Cells must be in suspension.

Since it is difficult to determine how many cells will be required to produce a monolayer on the slide, several dilutions of the sample are usually performed.  Since only a few hundred cells are required for most microscopic observations, the initial number of cells required is low, e.g., 5,000 to 10,000/ml in a total volume of 0.5 to 1.0ml.

Cells may be diluted in tissue culture medium, PBS, etc.

From 0.2 to 0.4 ml of cells is used for each cell dilution.

After centrifuging for approximately 5 min, the deposited cells are ready for fixation followed by subsequent staining.

Cells may be fixed and stained prior to centrifugation.
 

User Fees:

Please contact Dr. Raymond Hester at (334)460-6029.