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Biotechnical Services Core Facilities
located in the College of Medicine serve as a campus-wide resource providing
a variety of services and analyses available to researchers at the University
of South Alabama. The facilities are listed below.
Contact Information
Biopolymer
Laboratory:
The Biopolymer
Laboratory serves as a core facility for molecular biology research.
It is equipped with DNA synthesizers: ABI 381A, Beckman 1000M, DNA sequencer
373XL and Micro-array system Microsys 5100. Additional biotechnical
advice is provided to support a wide range of projects involving nucleic
acid research. Contact: Dr. Tin Cao, Director. Telephone
(251)460-7264; Fax (251)460-7931; email: tcao@jaguar1.usouthal.edu
Research
Cytometry Laboratory:
A Becton
Dickinson FACSVantage SE, dual laser flow cytometer is available for cell-surface
and intracellular antigen fluorescence phenotypic analysis, DNA measurement,
cell sorting and cloning, and kinetics analysis of Ca2+
modulation; forward and right angle light scatter and up to three colors
can be analyzed simultaneously. FACS doublet discrimination software insures
accuracy of cell cycle analysis. Two confocal laser scanning microscopes,
a Meridian ACAS 570 and a Leica TCS SP2, are available for fluorescence
analysis of tissue sections and adherent cells; up to three-colors can
be visualized simultaneously on the 3-laser TCS SP2 as well as volume rendering
(3-D reconstruction) of Z-sections. A TCS workstation with full LCS (Leica
confocal software) provides off-line data analysis. A third, non-confocal,
Olympus BX-50 fluorescence microscope is available for observation of such
standard fluorochromes as DAPI, FITC, and TRITC. Images from the
BX-50 are acquired with an Optronics cooled CCD camera; Photoshop 6.0 is
available for image analysis and a QMS Magicolor 2 for laser prints.
An Olympus SZX12 stereo zoom microscope is housed in the same laboratory.
A Molecular Devices Cytosensor microphysiometer is useful in signal transduction
detection and a PTI microscope-based photometer is capable of dual excitation/single
emission fluorescence detection. A Shandon cytocentrifuge is available
when techniques require suspended cells to be deposited on slides for subsequent
staining. The laboratory also houses tissue culture facilities including
a laminar flow hood, CO2
water-jacketed incubator, and centrifuge. Contact: Dr. Raymond
Hester, Director. Telephone (251)460-6029; Fax (251)460-6073; email:
rhester@jaguar1.usouthal.edu
Mass
Spectrometry Laboratory:
This facility
provides both consultation and analytical services primarily using a VG
70-250SEQ hybrid-tandem mass spectrometer, equipped with a HP 5890 gas
chromatograph. Contact: Dr. Ann Abraham, Telephone (251)460-7275;
Fax (251)460-6073; email: aabraham@jaguar1.usouthal.edu
Transgenic
Animal/ES Cell Core Laboratory:
This facility
provides assistance in the production of transgenic mouse models by either
DNA microinjection or gene targeting in embryonic stem (ES) cells. Contact:
Drs. Danna Zimmer or Warren Zimmer, Co-Directors. Telephone (251)460-6198;
Fax (251)460-6798; email: dzimmer@jaguar1.usouthal.edu
Biostatistics
and Epidemiology:
This core
unit is a resource for teaching, consulting, collaborating, and service
activities in health outcomes research, epidemiology, research methods
and design, biostatistics, database development and computer applications.
Contact: Dr. Donald Herbert, Director. Telephone (251)471-7828; Fax (251)471-7809;
email: dherbert@jaguar1.usouthal.edu
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